This is the first videogame about "The Web of Spider-Man" TV series.
- Black Cat
- Scarlet Spider
- Spider-Man 2099 (optianol Spidey bonus suit / unlockable after collecting 12 Spider-Trackers during the Rhino level without dying.)
- Doctor Octopus
- Molten Man
- Vulture (Final Boss / Ultimate Form)
The Vulture invades the museum of natural history and steals there new exhibit, The Tablet of Time! Spider-Man attacks Vulture who dodges, and he crashes into the display case. Vulture escapes, but Spidey webs his wing, pulling him forward and up into the air. Vulture lands on a building and the first boss battle is against him, but when he's about to get pictures, Vulture escapes and gains extra powers from the tablet wich fills his body with magic, Spidey chases him to the RAFT and Vulture with his now power frees everyone buy blasting The RAFT with his powers. Spidey must clear the city of the villains seeking revenge on Spidey.